No protective benefits of low dose acute L-glutamine supplementation on small intestinal permeability, epithelial injury and bacterial translocation biomarkers in response to subclinical exertional-heat stress: A randomised cross-over trial

Ogden, Henry B., Fallowfield, Joanne L., Child, Robert B., Davison, Glen, Fleming, Simon C., Delves, Simon K., Milyard, Alison, Westwood, Caroline S. and Layden, Joseph D. (2021) No protective benefits of low dose acute L-glutamine supplementation on small intestinal permeability, epithelial injury and bacterial translocation biomarkers in response to subclinical exertional-heat stress: A randomised cross-over trial. Temperature. ISSN 2332-8959 (In Press)

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Abstract

INTRODUCTION: Exertional-heat stress disrupts gastrointestinal permeability, and through subsequent bacterial translocation, can result in potentially fatal exertional-heat stroke. Glutamine supplementation is a potential countermeasure, although previously validated doses are not universally well-tolerated. METHODS: Ten males completed two 80-minute subclinical exertional-heat stress tests (EHST) following either glutamine (0.3 g·kg·FFM-1) or placebo supplementation. Small intestinal permeability was assessed using the lactulose/rhamnose dual-sugar absorption test and small intestinal epithelial injury using Intestinal Fatty-Acid Binding Protein (I-FABP). Bacterial translocation was assessed using total 16S bacterial DNA and Bacteroides/total 16S DNA ratio. RESULTS: The glutamine bolus was well tolerated, with no participants reporting symptoms of gastrointestinal intolerance. Small intestinal permeability was not influenced by glutamine supplementation (p = 0.06), though a medium effect size favouring the placebo trial was observed (d = 0.73). Both small intestinal epithelial injury (p < 0.01) and Bacteroides/total 16S DNA (p = 0.04) increased following exertional-heat stress, but were uninfluenced by glutamine supplementation. CONCLUSION: Acute low-dose oral glutamine supplementation does not protect gastrointestinal injury, permeability, or bacterial translocation in response to subclinical exertional-heat stress.

Item Type:	Article
Keywords:	Gut, Exercise, Endotoxin, I-FABP, Microbiota, Heat Stroke
Depositing User:	Ms Raisa Burton
Date Deposited:	14 Dec 2021 11:11
Last Modified:	14 Dec 2021 11:11
URI:	https://marjon.repository.guildhe.ac.uk/id/eprint/17671

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