Reliability of Gastrointestinal Barrier Integrity and Microbial Translocation Biomarkers at Rest and Following Exertional Heat Stress

Ogden, Henry B., Fallowfield, Joanne L., Child, Robert B., Davison, Glen, Fleming, Simon C., Edinburgh, Robert M., Delves, Simon K., Millyard, Alison, Westwood, Caroline S. and Layden, Joseph D. (2020) Reliability of Gastrointestinal Barrier Integrity and Microbial Translocation Biomarkers at Rest and Following Exertional Heat Stress. Physiological Reports, 8 (5). ISSN 2051-817X

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Official URL: https://doi.org/10.14814/phy2.14374

Abstract

Purpose: Exertional-heat stress adversely distrupts (GI) barrier integrity and, through subsequent microbial translocation (MT), negativly impacts health. Despite widespread application, the temporal reliability of popular GI barrier integity and MT biomarkers is poorly characterised. Method: Fourteen males completed two 80-minute exertional-heat stress tests (EHST) separated by 7-14 days. Venous blood was drawn pre, immediately- and 1-hour post both EHSTs. GI barrier integrity was assessed using the serum Dual-Sugar Absorption Test (DSAT), Intestinal Fatty-Acid Binding Protein (I-FABP) and Claudin-3 (CLDN-3). MT was assessed using plasma Lipopolysaccharide Binding Protein (LBP), total 16S bacterial DNA and Bacteroides DNA. Results: No GI barrier integrity or MT biomarker, except absolute Bacteroides concentration, displayed systematic trial order bias (p ≥ 0.05). I-FABP (trial 1 = Δ 0.834 ± 0.445 ng·ml-1; trial 2 = Δ 0.776 ± 0.489 ng·ml-1) and CLDN-3 (trial 1 = Δ 0.317 ± 0.586 ng·ml-1; trial 2 = Δ 0.371 ± 0.508 ng·ml-1) were increased post-EHST (p ≤ 0.01). All MT biomarkers were unchanged post-EHST. Coefficient of variation and typical error of measurement post-EHST were: 11.5% and 0.004 (ratio) for the DSAT 90-minutes post probe ingestion; 12.2% and 0.004 (ratio) at 150-minutes post probe ingestion; 12.1% and 0.376 ng·ml-1 for I-FABP; 4.9% and 0.342 ng·ml-1 for CLDN-3; 9.2% and 0.420 µg·ml-1 for LBP; 9.5% and 0.15 pg·µl-1 for total 16S DNA; and 54.7% and 0.032 for Bacteroides/total 16S DNA ratio. Conclusion: Each GI barrier integrity and MT translocation biomarker, except Bacteroides/total 16S ratio, had acceptable reliability at rest and post exertional-heat stress.

Item Type:	Article
Keywords:	Gut, Exercise, Endotoxin
Depositing User:	Ms Raisa Burton
Date Deposited:	04 Mar 2020 11:42
Last Modified:	10 Dec 2020 15:21
URI:	https://marjon.repository.guildhe.ac.uk/id/eprint/17536
Related URLs:	https://physoc. ... 4814/phy2.14374 (Publisher URL)

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